A novel technique has been developed to enhance single-cell genetic sequencing, allowing scientists to more effectively analyze the genomes of individual cells and environmental viral particles. This advancement is detailed in a study published in Nature Microbiology, conducted by researchers from Bigelow Laboratory for Ocean Sciences and Atrandi Biosciences. The method, termed environmental microcompartment genomics, was applied to surface seawater samples from the Gulf of Maine, demonstrating significant advantages over traditional sequencing methods, especially in exploring marine viruses.
The researchers were able to obtain genomic sequences from over 2,000 particles within just 300 nanoliters of seawater. This approach relies on advanced microfluidic technology that compartmentalizes samples into small bubbles, allowing random sampling of single cells and DNA amplification. Unlike conventional methods that require size-based sorting, this new technique processes particles of various sizes simultaneously.
Initial tests indicate that the process is also effective for sediment and soil samples. It offers a more comprehensive analysis of microbial communities without compromising data quality.
Funded by the National Science Foundation and other organizations, the study involved collaboration with scientists from Vilnius University and other institutions.
- Why it matters: This method can lead to more accurate environmental monitoring and a deeper understanding of microbial ecosystems, potentially influencing research, public health, and environmental management.
- The latest: The technique’s success in seawater paves the way for future applications in other environmental samples, enhancing the study of previously overlooked microbial populations.
Source: https://www.miragenews.com/new-method-broadens-virus-study-in-environment-1564381/
Source: https://www.miragenews.com/new-method-broadens-virus-study-in-environment-1564381/